rabbit polyclonal anti recoverin  (Proteintech)

Journal: International Journal of Molecular Sciences

Article Title: Targeting Oxidative Stress and Inflammation in the Eye: Insights from a New Model of Experimental Autoimmune Uveitis

doi: 10.3390/ijms252312910

Figure Lengend Snippet: Clinical characterization of recoverin-induced EAU in rabbits. ( A ) Representative photographs of the ocular fundus before (left) and 4 weeks after (right) immunization with recombinant bovine recoverin (7.5 mg/mL). Arrows indicate subretinal hemorrhages. ( B ) Scotopic ERG recordings performed before the immunization (left) and at week 4 after the procedure (right). ( C ) Amplitudes of the a-wave (left) and b-wave (right) of the electroretinogram at different time periods after recoverin administration. ( D ) Accumulation of antibodies against recoverin in sera according to ELISA. * p < 0.05 compared to the values obtained before immunization.

Article Snippet: The content of recoverin was evaluated by Western blotting of AH samples using rabbit polyclonal anti-recoverin antibodies (#DF3160, Affinity Biosciences, China; 1:1000 in PBST) and rat polyclonal anti-recoverin antibodies prepared as described in our previous study [ ] (1:10,000 in PBST).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Targeting Oxidative Stress and Inflammation in the Eye: Insights from a New Model of Experimental Autoimmune Uveitis

doi: 10.3390/ijms252312910

Figure Lengend Snippet: Histological characterization of recoverin-induced EAU in rabbits. Morphology of the posterior sector of the eye before ( A , B ) and 4 weeks after ( C , D ) immunization with recombinant bovine recoverin (7.5 mg/mL in PBS) mixed with Freund’s complete adjuvant (1:1). Inflammatory mononuclear cells infiltrating the vitreous body and retina ( D , short black arrows), Dalen–Fuchs nodules ( C , D , white arrows), the swelling of photoreceptors up to their fusion into homogeneous eosinophilic droplets ( D , red arrows), activation of the Müller glia ( D , red arrowheads) and areas of retinal atrophy ( C , D , long black arrows) are indicated. Retinal thickness in images ( C , D ) is altered relative to images ( A , B ) due to inflammatory and atrophic changes. Retinal fissures and detachment are of artificial origin. Staining by hematoxylin and eosin. Magnification: 200× (top row) or 400× (bottom row). Scale bar 100 µm on ( A ) (top row) and 50 µm on ( B ) (bottom row).

Article Snippet: The content of recoverin was evaluated by Western blotting of AH samples using rabbit polyclonal anti-recoverin antibodies (#DF3160, Affinity Biosciences, China; 1:1000 in PBST) and rat polyclonal anti-recoverin antibodies prepared as described in our previous study [ ] (1:10,000 in PBST).

Techniques: Recombinant, Adjuvant, Activation Assay, Staining

Journal: International Journal of Molecular Sciences

Article Title: Targeting Oxidative Stress and Inflammation in the Eye: Insights from a New Model of Experimental Autoimmune Uveitis

doi: 10.3390/ijms252312910

Figure Lengend Snippet: Biochemical changes in AH during the development of recoverin-induced EAU in rabbits. AH was collected before (control samples) and 3 and 4 weeks after immunization of the animals (experimental samples) using recombinant bovine recoverin (7.5 mg/mL in PBS mixed with Freund’s complete adjuvant 1:1) and analyzed for the total protein concentration ( A ), cytokine content ( B – D ), total antioxidant activity ( E ), antioxidant enzymes activities ( F , G ) and zinc level ( H ). * p < 0.05 compared to the values obtained for the control samples (before immunization).

Article Snippet: The content of recoverin was evaluated by Western blotting of AH samples using rabbit polyclonal anti-recoverin antibodies (#DF3160, Affinity Biosciences, China; 1:1000 in PBST) and rat polyclonal anti-recoverin antibodies prepared as described in our previous study [ ] (1:10,000 in PBST).

Techniques: Control, Recombinant, Adjuvant, Protein Concentration, Antioxidant Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Targeting Oxidative Stress and Inflammation in the Eye: Insights from a New Model of Experimental Autoimmune Uveitis

doi: 10.3390/ijms252312910

Figure Lengend Snippet: Changes in the lipid composition of AH during the development of recoverin-induced EAU. AH was collected before (control samples) and 3 and 4 weeks after immunization of the animals (experimental samples) using recombinant bovine recoverin (7.5 mg/mL in PBS mixed with Freund’s complete adjuvant 1:1) and analyzed for the signal lipid content. The identified lipid mediators were grouped according to their chemical families (PUFAs, oxylipins and phospholipid derivatives). Oxylipins were further classified according to their biosynthesis pathways involving COX, LOX or CYP. * p < 0.05 compared to the values obtained for the control samples (before immunization).

Article Snippet: The content of recoverin was evaluated by Western blotting of AH samples using rabbit polyclonal anti-recoverin antibodies (#DF3160, Affinity Biosciences, China; 1:1000 in PBST) and rat polyclonal anti-recoverin antibodies prepared as described in our previous study [ ] (1:10,000 in PBST).

Techniques: Control, Recombinant, Adjuvant

Journal: International Journal of Molecular Sciences

Article Title: Targeting Oxidative Stress and Inflammation in the Eye: Insights from a New Model of Experimental Autoimmune Uveitis

doi: 10.3390/ijms252312910

Figure Lengend Snippet: Changes in AH metabolome during the development of recoverin-induced EAU. AH was collected before (control samples) and 3 and 4 weeks after immunization of the animals (experimental samples) using recombinant bovine recoverin (7.5 mg/mL in PBS mixed with Freund’s complete adjuvant 1:1) and analyzed for core metabolites. The identified compounds are presented in alphabetical order. * p < 0.05 compared to the values obtained for the control samples (before immunization).

Article Snippet: The content of recoverin was evaluated by Western blotting of AH samples using rabbit polyclonal anti-recoverin antibodies (#DF3160, Affinity Biosciences, China; 1:1000 in PBST) and rat polyclonal anti-recoverin antibodies prepared as described in our previous study [ ] (1:10,000 in PBST).

Techniques: Control, Recombinant, Adjuvant

Journal: International Journal of Molecular Sciences

Article Title: Targeting Oxidative Stress and Inflammation in the Eye: Insights from a New Model of Experimental Autoimmune Uveitis

doi: 10.3390/ijms252312910

Figure Lengend Snippet: Leakage of recoverin into AH during EAU development. AH was collected before (control samples) and 3 and 4 weeks after immunization of the animals (experimental samples) using recombinant bovine recoverin (7.5 mg/mL in PBS mixed with Freund’s complete adjuvant 1:1) and analyzed for the presence of recoverin (Rec) by Western blotting (top). The amounts of recoverin in AH were calculated by densitometric analysis of the protein bands (bottom). * p < 0.05 compared to the values obtained for the control samples (before immunization).

Article Snippet: The content of recoverin was evaluated by Western blotting of AH samples using rabbit polyclonal anti-recoverin antibodies (#DF3160, Affinity Biosciences, China; 1:1000 in PBST) and rat polyclonal anti-recoverin antibodies prepared as described in our previous study [ ] (1:10,000 in PBST).

Techniques: Control, Recombinant, Adjuvant, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Targeting Oxidative Stress and Inflammation in the Eye: Insights from a New Model of Experimental Autoimmune Uveitis

doi: 10.3390/ijms252312910

Figure Lengend Snippet: Parameters of recoverin-induced EAU under mitochondria-targeted antioxidant therapy. Treatment with 7.5 μM SkQ1 was performed 3 times daily for 3 days before immunization and 3 times daily for 3 or 4 weeks after immunization with recoverin. AH was collected in the groups with or without treatment before and 3 and 4 weeks after immunization. The samples were subjected to biochemical examination, as described in and . Results of the analysis of antioxidant activity ( A ), as well as TNF-α ( B ), IL-6 ( C ) and PGE2 ( D ) concentrations, are presented. ( E , F ) Scotopic ERG recordings ( E ) and morphology of the posterior sector of the eye ( F ; hematoxylin and eosin staining, magnification 400×, scale bar 50 μm) at week 4 after immunization with or without treatment. * p < 0.05 compared to the corresponding values obtained for samples from the group without treatment.

Article Snippet: The content of recoverin was evaluated by Western blotting of AH samples using rabbit polyclonal anti-recoverin antibodies (#DF3160, Affinity Biosciences, China; 1:1000 in PBST) and rat polyclonal anti-recoverin antibodies prepared as described in our previous study [ ] (1:10,000 in PBST).

Techniques: Antioxidant Activity Assay, Staining

Journal: iScience

Article Title: The RBPMS CreERT2-tdTomato mouse line for studying retinal and vascular relevant diseases

doi: 10.1016/j.isci.2023.108111

Figure Lengend Snippet: Expression of RBPMS in adult RBPMS CreERT2-tdTomato mouse ocular tissue (A) Representative image of an adult mouse ocular frozen section after tamoxifen induction with strong tdTomato fluorescence signals present in the retinal ganglion layer (RGL), optic nerve, choroid vasculature and ciliary body. Scale bar, 500 μm. (B and C) Ocular frozen sections labeled with RBPMS by immunofluorescent staining (green), and the ratio of tdTomato + and RBPMS + cells. Data are represented as mean ± SEM. Scale bar, 100 μm. (D and E) Ocular frozen sections labeled with Tuj-1 by immunofluorescent staining (green), and the ratio of tdTomato + and Tuj-1 + cells. Data are represented as mean ± SEM. Scale bar, 100 μm. (F and G) Ocular frozen sections labeled with Calretinin by immunofluorescent staining (green), and the ratio of tdTomato + and Calretinin + cells. Data are represented as mean ± SEM. Scale bar, 100 μm. (H) The ocular frozen sections were labeled with tdTomato by immunofluorescent staining (green). The right images show a higher magnification of the corresponding white box area. Scale bar, 100 μm. (I) Ocular frozen sections were labeled with VSX2 by immunofluorescent staining (pseudo color green), Scale bar, 100 μm. (J) The ocular frozen sections were labeled with Recoverin by immunofluorescent staining (green), Scale bar, 100 μm.

Article Snippet: Rabbit polyclonal anti-Recoverin , Sigma-Aldrich , Cat# AB5585; RRID: AB_3064861.

Techniques: Expressing, Fluorescence, Labeling, Staining

Journal: iScience

Article Title: The RBPMS CreERT2-tdTomato mouse line for studying retinal and vascular relevant diseases

doi: 10.1016/j.isci.2023.108111

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Recoverin , Sigma-Aldrich , Cat# AB5585; RRID: AB_3064861.

Techniques: Recombinant, Lysis, Protease Inhibitor, Plasmid Preparation, Software

Journal: Eye and Vision

Article Title: Microglia activation and neuronal alterations in retinas from COVID-19 patients: correlation with clinical parameters

doi: 10.1186/s40662-023-00329-2

Figure Lengend Snippet: Primary and secondary antibodies used in this study

Article Snippet: Recoverin , Rabbit polyclonal , Proteintech (10073-1-AP) , 1:500.

Techniques: Marker

Journal: PLoS ONE

Article Title: Functional and Molecular Characterization of Rod-like Cells from Retinal Stem Cells Derived from the Adult Ciliary Epithelium

doi: 10.1371/journal.pone.0033338

Figure Lengend Snippet: RNS-derived rods were analyzed after 40 days of differentiation. Rho protein at the cell surface was detected with two different monoclonal antibodies: 1D4 ( A ) and RET-P1 ( B ). The magnification in A shows an example of a Rho + cell labeled with 1D4 recognizing an intracellular epitope and requiring permeabilization of the sample. The antibody recognizing an extracellular epitope (RET-P1) and therefore detecting the protein at the cell surface (B) is characterized by a different labeling compared to the staining with 1D4 (A). ( B ) Double-labeling with antibodies for Rho (red) and Transducin (green). ( C ) Double-labeling with antibodies for Rho (red, RET-P1) and Recoverin (green). ( D ) Double-labeling with antibodies for Rho (red, 1D4) and peripherin (green). ( E ) Double-labeling with antibodies for Rho (red, 1D4) and Pde6b (green). Scale bars are 50 µm. Scale bar for C, D and E are equal to that shown in B. ( F ) Time course experiment analyzing RNS-derived cells co-expressing either Rho (1D4)+Pde6b (blue bars) or Rho (RET-P1)+Transducin (red bars) at different times of differentiation in vitro . Data are represented as mean +/− s.e.m. and derived from 10 fields from 2 independent experiments. On the right-hand side we show mRNA for Rho , Pde6b , Gnat1 (transducin) and S26 detected by RT-PCR at D20 and D30 confirming the expression these genes. ( G ) Real-time PCR for Cnga1 mRNA shows an increase in RNS-derived cells after 20 (D20) and 30 (D30) days of differentiation compared to expression in RNS (set as 0). ( H ) Real-time PCR for Gucy2f and Guca1 show up-regulation of these genes during differentiation. Data derive from the formula 2 −ΔCt .

Article Snippet: Primary antibodies were used as follows: 1∶100 anti-rhodopsin mouse monoclonal 1D4 (Sigma-Aldrich), 1∶1000 anti-rhodopsin mouse monoclonal RET-P1 (Sigma-Aldrich), 1∶100 anti-Pde6b rabbit polyclonal (ABCAM, Cambridge, UK), 1∶100 anti-recoverin rabbit polyclonal (Millipore), 1∶100 anti-peripherin rabbit polyclonal (Millipore), 1∶300 anti-transducin rabbit polyclonal (Sigma-Aldrich), 1∶100 anti-AIF rabbit polyclonal (Sigma-Aldrich).

Techniques: Derivative Assay, Labeling, Staining, Expressing, In Vitro, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction